Test for total aerobic microbial count

Total aerobic microbial count is one type of microbiological purity testing, which is used to count the number of colony forming unit present in an article complying with monograph standards. PREPARATION OF CULTURE MEDIA: Sodium hydroxide 1N: Dissolve 4.0 g of sodium hydroxide in water to make 100 mL.
Hydrochloric Acid 1N: Dissolve 8.5 ml of Hydrochloric Acid in water to make 100 mL. Soyabean-Casein Digest Broth Medium (SCDM)/ Soyabean-Casein Digest Agar(SCDA)/ Plate Count Agar(PCA): Reconstitute dehydrated media as directed by the manufacturer and sterilize in an autoclave at 121°C for 20 minutes.
AEROBIC MICROBIAL, General Testing Procedure

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Preparation of barium chloride solution

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 10.0 grams of Barium Chloride LR and transfer slowly, into above said beaker, through it‘s side walls, while gentle mixing, by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Barium Chloride , Distilled water, Glass rod, Glass funnel, Calibrated Analytical Balance

Procedure for Collection Transfer and Disposal of Scrap

Scrap essentially consists of metal scrap (MS, SS, Aluminum etc.) arising from engineering fabrication work and fiber drums, HDPE drums, carboys and polythene covers etc. from raw material packaging.Collection of Scrap: Scrap collection points are identified at the following locations. Scrap arising are placed in the above collection points. Scrap from these points are periodically shifted to scrap disposal yard outside Scrap segregation is done at disposal yard – namely as MS, SS, Aluminum, HDPE drums, carboys, fiber drums, polyethylene bags and cardboard cartons. Scrap is disposed from the disposal yard. Collection , Transfer, Disposal of Scrap, segregation

PREPARATION OF SILVER NITRATE SOLUTION (5 percent )

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Silver Nitrate LR and transfer slowly, into above-said beaker, through its sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 5.0% w/v. Silver Nitrate , Glass funnel, Beaker, Distilled water , Glass rod

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