Total aerobic microbial count is one type of microbiological purity testing, which is used to count the number of colony forming unit present in an article complying with monograph standards. PREPARATION OF CULTURE MEDIA: Sodium hydroxide 1N: Dissolve 4.0 g of sodium hydroxide in water to make 100 mL.
Hydrochloric Acid 1N: Dissolve 8.5 ml of Hydrochloric Acid in water to make 100 mL. Soyabean-Casein Digest Broth Medium (SCDM)/ Soyabean-Casein Digest Agar(SCDA)/ Plate Count Agar(PCA): Reconstitute dehydrated media as directed by the manufacturer and sterilize in an autoclave at 121°C for 20 minutes.
AEROBIC MICROBIAL, General Testing Procedure
Hydrochloric Acid 1N: Dissolve 8.5 ml of Hydrochloric Acid in water to make 100 mL. Soyabean-Casein Digest Broth Medium (SCDM)/ Soyabean-Casein Digest Agar(SCDA)/ Plate Count Agar(PCA): Reconstitute dehydrated media as directed by the manufacturer and sterilize in an autoclave at 121°C for 20 minutes.
AEROBIC MICROBIAL, General Testing Procedure
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