Operation Cleaning And Calibration Of Bio Safety Cabinet

Connect the power plug to the socket. Check the manometer reading initially that oil level at zero. Put on the power switch to start up. Manometer reading should not be less than 10mm water gauge scale while laminar airflow is running Enter the details of manometer reading in Annexure –I.
There are four switches and a pressure manometer at the left side panel of the instrument. Switch (1) for air flow. Switch (2) for tube light. Switch (3) for Exhaust. Switch (4) UV light. Put ON the switch (1) to start the airflow and manometer no –2 will show the reading of Airflow.
Put ON switch (2) to start normal light. Put on the Switch (3) for Exhaust and the manometer No –1 will show the reading of the exhaust. Put on the Switch (4) for UV Light.
Operation, Microbiology, Calibration, Cleaning

Comments

Preparation of barium chloride solution

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 10.0 grams of Barium Chloride LR and transfer slowly, into above said beaker, through it‘s side walls, while gentle mixing, by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Barium Chloride , Distilled water, Glass rod, Glass funnel, Calibrated Analytical Balance

preparation of sodium hydroxide solution dilute

Pour about 75 ml of distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Sodium Hydroxide Pellets LR and transfer slowly, into above said beaker, through it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask by using a glass funnel. Rinse the beaker and funnel with few ml of distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 5.0% w/v. Sodium Hydroxide Pellets , glass funnel, sidewalls, glass rod

Operation of Gas Chromatograph

To lay down the procedure for operation of gas chromatograph (SHIMADZU GC-2010 with AOC-5000) system. Shimadzu gc system, gc-2010. Aoc 5000 headspace sampler and auto-injector. 3gc solution software. Ensure that the working bench and area is clean. Always ensure gas line pressure is maintained above 4kg/cm2 in all gas supply lines . Gas Chromatograph , tighten, injection , magnetic force

ICHAPPS

Search This Blog