Pick the individual colonies from the previously exposed plates in which growth found. Purify the colonies, if necessary by streaking on to Soybean casein digest agar plate. Observe and record the morphological characters of the colonies such as shape, elevation, color and margins in Bacterial isolate identification as per annexure-I. Using sterile inoculating technique, inoculate the organism on two Soybean casein digest agar slants.
Use one slant culture to determine the cultural characteristics and the other to repeat any of the tests, if necessary as stock. Make a smear of the colony by taking with a loop and subject it to Gram’s Staining procedure. Observe under a microscope and note the reaction, morphology and arrangement of the cells.
Procedure, Identification, Bacterial Isolate, Microbiology
Use one slant culture to determine the cultural characteristics and the other to repeat any of the tests, if necessary as stock. Make a smear of the colony by taking with a loop and subject it to Gram’s Staining procedure. Observe under a microscope and note the reaction, morphology and arrangement of the cells.
Procedure, Identification, Bacterial Isolate, Microbiology
Comments
Post a Comment