Procedure for Operation Cleaning and Calibration of BOD Incubator

Connect power plug to the socket. Put ON the Mains on the control panel, temperature controller displays and the inside chamber motor ON. Set the thermostat at 5°C above the Set Temperature on the temperature controller. The Safety thermostat will cut off the mains contactor in case of overshoot of the temperature by 5°C.
Set the Print time interval for 30 minutes on PC /Printer interface unit and connect the same to 80 column printer or to the PC for data communication. Put ON the Heating Switch on the Control Panel, Dry heater will be ON.
Put ON the Cooling Switch on the Control Panel, Refrigeration condenser motor and compressor will be ON. Enter the temperature into Temperature Monitoring record as per Annexure – I. The temperature should not vary? 1°C against the set temperature
Procedure, Operation, Calibration, BOD Incubator, Microbiology

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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask

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