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Environmental Monitoring By Plate Exposing

Prepare Soybean Casein Digest agar (SCDA) and Sabouraud Dextrose Agar (SDA) as per current version of GTP No.- XXXXX. Aseptically dispense the media in Petri dishes. After solidification invert and incubate the plates for 24 hours at 35°C. Carry the plates in the Petri cans for plate exposing to the respective area.
Follow the entrance procedure available at that place and enter into the respective area accordingly.  Mark the Plates as per Point No given in Appendix’s according to respective block before wearing gloves. Wear the hand gloves and clean the surface where plate has to be exposed by 70% isopropyl alcohol. Open the plate carefully and keep the lid beside.
Expose the plate as per Appendix – I, II, III, IV, V, and VI layouts. Expose the plates for 120 minutes. After completion closes the plate by a lid. After exposing carry the plates in Petri cans to the microbiology lab.
Environmental, Microbiology, Monitoring, Plate Exposing


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