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Operation and calibration of ( gradient hplc shimadzu class vp series )

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BASIC OPERATION : Switch ‘ON’ the main for LC-10 pump. Switch ‘ON’ the main for auto injector. Switch ‘ON’ the main for UV vis detector. Switch ‘ON’ the system controller and the computer.
After switching ‘ON’ all the components, finally switch ‘ON’ the computer. Default screen appears on the respective screen modules. Double click on the (Shimadzu class VP) icon in the screen. Double click on Instrument 1 icon.
Here for the beep sound, which indicates the communication between the system and the software. OPERATING PROCEDURE FOR LC-10AVP PUMP Open the drain valve of the pump.  Press the purge key.  Ensure that there are no air bubbles in the flow line and press purge/pump key to stop purging.  Close the drain valve of the pump.
Operation, Calibration, Shimadzu, GRADIENT


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