Operation and calibration of ( gradient hplc shimadzu class vp series )

BASIC OPERATION : Switch ‘ON’ the main for LC-10 pump. Switch ‘ON’ the main for auto injector. Switch ‘ON’ the main for UV vis detector. Switch ‘ON’ the system controller and the computer.
After switching ‘ON’ all the components, finally switch ‘ON’ the computer. Default screen appears on the respective screen modules. Double click on the (Shimadzu class VP) icon in the screen. Double click on Instrument 1 icon.
Here for the beep sound, which indicates the communication between the system and the software. OPERATING PROCEDURE FOR LC-10AVP PUMP Open the drain valve of the pump. Press the purge key. Ensure that there are no air bubbles in the flow line and press purge/pump key to stop purging. Close the drain valve of the pump.
Operation, Calibration, Shimadzu, GRADIENT

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Preparation of barium chloride solution

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Scrap essentially consists of metal scrap (MS, SS, Aluminum etc.) arising from engineering fabrication work and fiber drums, HDPE drums, carboys and polythene covers etc. from raw material packaging.Collection of Scrap: Scrap collection points are identified at the following locations. Scrap arising are placed in the above collection points. Scrap from these points are periodically shifted to scrap disposal yard outside Scrap segregation is done at disposal yard – namely as MS, SS, Aluminum, HDPE drums, carboys, fiber drums, polyethylene bags and cardboard cartons. Scrap is disposed from the disposal yard. Collection , Transfer, Disposal of Scrap, segregation

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Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Silver Nitrate LR and transfer slowly, into above-said beaker, through its sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 5.0% w/v. Silver Nitrate , Glass funnel, Beaker, Distilled water , Glass rod

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