Operation Cleaning And Calibration Of Single Door Autoclave

Check the water level before starting the autoclave. If the level is down, then add purified water upto the level mark. Open the door and load the necessary items to be sterilized as required.
Place the autoclave indicator strips at different places in the autoclave for each cycle to indicate the proper sterilization by color differentiation of the strip.
Close the door using the radial locking handles clockwise till it is tight. Switch on the mains to supply the power followed by the toggle switch located in the control panel to activate the heat generator.
Operation, Cleaning, Calibration, Single Door Autoclave, Microbiology

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Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask

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