Operation of ULTRASOINC BATH FLEXIT

Switch on the instrument using the RED switch which is on the front of the instrument. Set the desired time for sonication from 0.00 minutes to 9.59 minutes by using the upward arrow keys on the micro timer panel. Start/Stop the sonication by pressing the RED color round toggle key.
Pour Demineralized water in the ultrasonic bath. Drain the water in the ultrasonic bath daily or whenever any material is spilled or whenever the color of water changes. Ensure the water level at the time of sonication to avoid the floating of the bottles used.
Ultrasonic Bath, micro timer panel, sonication, soft cloth

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Preparation of barium chloride solution

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 10.0 grams of Barium Chloride LR and transfer slowly, into above said beaker, through it‘s side walls, while gentle mixing, by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Barium Chloride , Distilled water, Glass rod, Glass funnel, Calibrated Analytical Balance

preparation of sodium hydroxide solution dilute

Pour about 75 ml of distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Sodium Hydroxide Pellets LR and transfer slowly, into above said beaker, through it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask by using a glass funnel. Rinse the beaker and funnel with few ml of distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 5.0% w/v. Sodium Hydroxide Pellets , glass funnel, sidewalls, glass rod

Preparation of nitric acid dilute (10 percent)

Measure 10.6 ml of Nitric Acid LR and transfer into a 50.0 ml capacity beaker. Pour about 75.0 ml of distilled water into a 250 ml capacity beaker. Transfer slowly, through sidewalls of the beaker, 10.6 ml of Nitric Acid LR into above said beaker, while gentle mixing, by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask by using a glass funnel. Rinse the beakers and funnel with few ml of distilled water , and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Nitric Acid , Glass rod, Glass funnel, Beaker, Volumetric flask , Measuring cylinders

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