Calibration Of HPLC Waters System

C ALIBRATION OF PUMP: Accuracy of flow rate: Fill filtered water in the pump reservoir and purge the pump. Set the Flow rate of the pump to 1.0 ml/minute and ensure no air bubble is stuck up in the path. Take a clean 10 ml Class – A graduated cylinder. Note the time and immediately start collecting water into the 10 ml graduated cylinder.
Calibration, Equipment, graduated cylinder, concentration