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Calibration Of HPLC Waters System

C ALIBRATION OF PUMP: Accuracy of flow rate: Fill filtered water in the pump reservoir and purge the pump. Set the Flow rate of the pump to 1.0 ml/minute and ensure no air bubble is stuck up in the path. Take a clean 10 ml Class – A graduated cylinder. Note the time and immediately start collecting water into the 10 ml graduated cylinder.
Calibration, Equipment, graduated cylinder, concentration

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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask