Ensure that the laminar airflow is working. Place a drop of sterile water/purified water on the grease free glass slide. Scrap a colony (if growth is on solid media) or take a loopful from the suspension with the help of inoculating wire loop (nichrome/platinum) and Prepare a smear on the grease free glass slide by rotating the loop.
Allow to air dry the smear and heat fix it by passing the slide gently through a flame. Flood/cover the smear with Gram’s Crystal violet solution for 1 min exactly. Rinse the stained slide with tap water and drain off excess. Flood the smear with Gram’s Iodine solution for 1 min.
Procedure, Staining, Microbiology, microorganism
Allow to air dry the smear and heat fix it by passing the slide gently through a flame. Flood/cover the smear with Gram’s Crystal violet solution for 1 min exactly. Rinse the stained slide with tap water and drain off excess. Flood the smear with Gram’s Iodine solution for 1 min.
Procedure, Staining, Microbiology, microorganism
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