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Thin Layer Chromatography

In thin-layer chromatography, the adsorbent is a relatively thin, uniform layer of dry, finely powdered material applied to a glass, plastic, or metal sheet or plate, glass plates and aluminum plates being most commonly employed. The separation achieved may be based upon adsorption, partition, or a combination of both effects, depending on the particular type of stationary phase, its preparation, and its use with different solvents. General Testing Procedure, Procedure, chromatography, thin layer chromatography



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Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask