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OPTICAL ROTATION

OPTICAL ROTATION: Substances that may show optical rotatory power are chiral. Those that rotate light in a clockwise direction as viewed towards the light source are dextrorotatory, or (+) optical isomers. Those that rotate light in the opposite direction are called levorotatory or (–) optical isomers.
SPECIFIC ROTATION: The specific rotation of a chemical compound [α] is defined as the observed angle of optical rotation α, when plane-polarized light is passed through a sample with a path length of 1 decimeter and a sample concentration of 1 gram per 1 deciliter. The specific rotation of a pure material is an intrinsic property of that material at a given wavelength and temperature.
General Testing Procedure, OPTICAL, ROTATION


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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask