Ensure that the system is connected to stabilized power supply. Put on the main switch of the instrument. Identify the column to be used for the Analysis and make relevant entries in the HPLC column register.
Connect the prescribed column in the right direction, connect the tubing from the injector to one end of the column and other ends to the tubing from the detector.
Agilent, Thermostatted, autosampler, Pump, Set up injector
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