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Injection Sequence For Chromatography

To have a procedure to follow the injection sequence for HPLC and GC analysis. This SOP is applicable to the HPLC and GC analysis in Quality Control Department. It is the responsibility of  Quality Control personnel to follow this SOP.
Ensure that the chromatographic conditions are as per the Method / SOP pertaining to the particular product. Inject the mobile phase or diluent in which the sample is to be dissolved as blank run.
injection, sequence, chromatography
ich

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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask