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Calibration of HPLC

To lay down the procedure for calibration of hplc. This sop is applicable for the calibration of hplc’s in qc / ipqc laboratories. Follow the respective sop for operation and carry out the following tests for the calibration of a hplc system as applicable.
Flow rate accurac, gradient composition acuracy, injection volume precision, oven temperature/sample chiller temperature.
calibration of HPLC, Restriction capillary, specific gravity, separately, caffeine
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