Procedure For Operation And Maintenance Of Hot Plate

To lay down the Procedure for Operation and Maintenance of Hot Plate. The responsibility of operating the hot plate as per procedure lies with Microbiologist /Analyst.
Hotplate having Heater Indicating lamp, 3 heat switch control knob on left hand side and Thermostat knob on right hand side.
Hot Plate, Microbiology, Heater Indicating lamp
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Preparation of barium chloride solution

Pour about 75 ml of Distilled water into a 250 ml capacity beaker. Weigh 10.0 grams of Barium Chloride LR and transfer slowly, into above said beaker, through it‘s side walls, while gentle mixing, by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Barium Chloride , Distilled water, Glass rod, Glass funnel, Calibrated Analytical Balance

preparation of sodium hydroxide solution dilute

Pour about 75 ml of distilled water into a 250 ml capacity beaker. Weigh 5.0 grams of Sodium Hydroxide Pellets LR and transfer slowly, into above said beaker, through it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask by using a glass funnel. Rinse the beaker and funnel with few ml of distilled water, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with distilled water, remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 5.0% w/v. Sodium Hydroxide Pellets , glass funnel, sidewalls, glass rod

Operation of Gas Chromatograph

To lay down the procedure for operation of gas chromatograph (SHIMADZU GC-2010 with AOC-5000) system. Shimadzu gc system, gc-2010. Aoc 5000 headspace sampler and auto-injector. 3gc solution software. Ensure that the working bench and area is clean. Always ensure gas line pressure is maintained above 4kg/cm2 in all gas supply lines . Gas Chromatograph , tighten, injection , magnetic force

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