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Procedure For Handling Of Medias To Be Followed For Receiving Preparation Growth Promotion Test Storage Destruction Of Media

To lay down the procedure for Handling of Medias to be followed for Receiving, Preparation, Growth promotion test, Storage, Destruction of media.
This procedure is applicable for receiving, preparation, growth promotion test, storage and destruction of every lot / batch of media and also for destruction
Hydrochloric acid, 1N Sodium Hydroxide, Microbiolog
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Preparation of mercuric acetate solution 10 percent

Pour about 75 ml of Glacial Acetic Acid into a 250 ml capacity beaker. Weigh 10.0 grams of Mercuric Acetate LR and transfer slowly, into above-said beaker, though it‘s sidewalls, while gentle mixing by using a glass rod. Transfer prepared solution into a 100 ml volumetric flask, by using a glass funnel. Rinse the beaker and funnel with few ml of Glacial Acetic Acid, and allow to add into the contents of volumetric flask. Make up the volumetric flask to mark with Glacial Acetic Acid , remove the funnel, keep the lid to close the volumetric flask and shake gently to mix the solution. Transfer the prepared solution into a suitable container and label with all necessary details. The resultant solution is having the concentration of 10.0% w/v. Mercuric Acetate , Glacial Acetic Acid , Glass funnel, Volumetric flask