ICHAPPS

Dissolve or dilute 10 g or 10 ml of the preparation being examined, unless otherwise prescribed, in Lactose broth or another suitable medium shown not to have anti-microbial activity under the conditions of the test and adjust the volume to 100 ml with the same medium. If necessary, adjust the pH to about 7.0 ± 0.2. Staphylococcus aureus , Bacteriological Incubator, Analytical Balance

d being examined, unless otherwise prescribed, in Lactose broth or another suitable medium shown not to have anti-microbial activity under the conditions of the test and adjust the volume to 100 ml with the same medium. If necessary, adjust the pH to about 7.0 ± 0.2. Take 10 ml or 10 g, the quantity corresponding to 1.0 g or 1.0ml (as per the specification) of the pretreated sample in a suitable vessel. Autoclave , General testing procedure,  Laminar Air flow , BOD – Incubator

Dissolve or dilute 10 g or 10 ml of the preparation being examined, unless otherwise prescribed, in Lactose broth or another suitable medium shown not to have anti-microbial activity under the conditions of the test and adjust the volume to 100 ml with the same medium If necessary, adjust the pH to about 7.0 ± 0.2. Blue Agar , Eosin-Methylene , General testing procedure,

Stock Solution : Dissolve 34 g of Monobasic Potassium Phosphate in about 500 ml of water contained in a 1000 ml volumetric flask. Adjust to pH 7.2 ± 0.1 by the addition of 4 % w/v aqueous solution of Sodium Hydroxide (about 175 ml), add water to volume, and mix. Dispense and sterilize an Autoclave at 121–1240C, 1.1psi pressure for about 30 min. Store under refrigeration. Aerobic Microbial Count , General testing procedure, incubated

Weigh 58.09 g of media in a butter paper using a clean weighing balance. Transfer the weighed media into clean bottle/beaker containing 1000 ml of distilled water/ Purified water. Adjust the final pH (at 250C) 6.9 ± 0.2. Heat to dissolve the medium completely by keeping the beaker/bottle on the hot plate. Sterilize the media bottle in an autoclave at 121–1240C for 30 minutes. Avoid over heating of the media. After sterilization cool the media to 40 – 500C. Dehydrated , General testing procedure, pH – meter, Hot air Oven

To lay down the procedure for Operation, Calibration and Maintenance of Non - contact Infrared Thermometer. The responsibility of operation, calibration and maintenance of Non-contact Infrared Thermometer lies with microbiologist/Analyst. Switch on the instrument by pressing the ENT button (red switch present at handle). The display will show the temperature and the laser will turn on. Microbiology, Thermometer , static electricity

To lay down the Procedure for Operation of Microscope.  The responsibility of operating the Microscope as per procedure lies with Microbiologist. Place a slide over the stage, specimen side up and center of the specimen to be examined as accurately as possible over the hole in the center of the stage with the help of slide mover. Microscope , Microbiology, diameter

To lay down the procedure for operation and calibration of pH meter. The responsibility of operation and calibration of pH meter lies with the Microbiologist /Analyst. The pH meter consists of a single electrode capable of reading the pH from 2-13 range, and ATP probe capable of reading the temperature from 0-100° C. pH Meter , Microbiology, filter paper

To lay down the Procedure for Operation of UV Pass Box and its usage. This procedure applies to UV pass box located between Testing room and Sterilization room used for transfer of materials from sterilization room into the testing area and Washing room and Incubator room used for transfer of materials from incubator room into washing area. UV Pass Box , Microbiology, visual indicators

To lay down the Procedure for Operation and Maintenance of Hot Plate.  The responsibility of operating the hot plate as per procedure lies with Microbiologist /Analyst. Hotplate having Heater Indicating lamp, 3 heat switch control knob on left hand side and Thermostat knob on right hand side. Hot Plate , Microbiology, Heater Indicating lamp

To lay down the Procedure for the Operation of Digital Colony Counter.  Person from concerned department directly involved in the activity shall be responsible for operation of digital colony counter. Clean the surface of the wolffhuegel glass and keep the petridish on it. Ensure that the plug of the auto marker pen is connected to the probe socket provided. Microbiology, Colony Counter , Operation, purified water

To lay down the procedure for operation, calibration and temperature mapping of horizontal and vertical autoclave. The responsibility of operation and calibration of horizontal and vertical autoclave lies with the microbiologist. Open the door of the autoclave manually by rotating lever at non-sterile end. sterilization cycle , Microbiology, horizontal, vertical autoclave , temperature

To lay down the procedure for Handling of Medias to be followed for Receiving, Preparation, Growth promotion test, Storage, Destruction of media. This procedure is applicable for receiving, preparation, growth promotion test, storage and destruction of every lot / batch of media and also for destruction Hydrochloric acid , 1N Sodium Hydroxide, Microbiolog

To lay down the procedure for operation and calibration of oxygen analyzer and anaerobic chamber. This procedure is applies to the operation and calibration of oxygen analyzer and anaerobic chamber in microbiology lab. Person from concerned department directly involved in the activity shall be responsible for operation and calibration of oxygen analyzer and anaerobic chamber . Microbiology, oxygen analyzer , anaerobic chamber

To lay down the Procedure for Operation and Calibration of Lab Oven. This procedure applies to operation and calibration of lab oven used for sterilization of lab accessories. The responsibility of operation and calibration of lab oven lies with Microbiologist/Analyst. Microbiology, Calibration of Lab Oven , sterilization

To lay down the procedure to be followed for the operation, calibration and maintenance of Air sampler. The responsibility of operation and calibration of himedia Air sampler and MAS-100 NT Air sampler lies with Microbiologist / Analyst. Microbiology , procedure, operation, Air sampler

To lay down the procedure for operation, calibration and maintenance of incubators. This procedure applies to incubators used in microbiology for incubation at required temperatures respectively with the following details. The responsibility of proper operation, calibration and maintenance of incubators lies with the Microbiologist. Microbiology, incubators , calibration, temperature

To lay down the procedure for environmental monitoring of bio-load in controlled areas by using settle plates, air sampling and contact plate. This procedure applicable to controlled areas like pharma areas at different locations where crystallization, centrifuging , vacuum drying, sifting, blending, micronisation, compaction and packing of finished product are carried out and Microbiology testing area. settle plates , contact plate, environmental, procedure, Microbiology

To lay down the procedure for microbial analysis of finished product (API). This procedure applicable to test the total aerobic microbial count and total combined yeast and molds count and to test for the presence of pathogens in any finished product (API). Microbiology, finished product, microbial analysis , 1N NaoH, 1N Hcl

To lay down the procedure for sampling, testing and release of potable water and purified water. This procedure applicable to be followed during sampling, testing and release of potable water and purified water. The responsibility of s ampling and testing of potable wate r and purified water lies with the Quality Control analyst and release of potable water and purified water lies with QCD-In charge or his designate. sampling, testing , Microbiology, potable water , purified water

To lay down the procedure for Investigation of out of specification (OOS) results in Microbiology. This procedure is applicable to Investigation of out of specification (OOS) results in microbial testing of Non-Sterile Products , Water Samples, and Environmental Monitoring carried at Astrix Laboratories Limited, Kazipally. Microbiology , specification, Investigation

To lay down the procedure for conducting the Bacterial Endotoxin Test of Purified Water and Operation of Cyclomixer. This procedure applies to testing of bacterial endotoxins for purified water by gel clot method and also applies to operation of cyclomixer. The responsibility of performing the bacterial endotoxin test for purified water and operation of cyclomixer lies with Microbiologist/Analyst. Microbiology, conducting, Bacterial Endotoxin , Purified Water, Cyclomixer

To lay down the procedure to be followed for cleaning of glassware and sterilization glassware and microtips. This procedure applies to cleaning of glassware and sterilization of glassware and microtips used for microbiological testing . The responsibility of cleaning and sterilization of glassware and micro tips lies with Microbiologist/Analyst. Microbiology , cleaning, sterilization glassware , microtips

To lay down the Procedure to test the efficacy of UV lamp against microbial challenge. This procedure applies to UV lamps of Pass box, Laminar Air Flow bench and Garments box located in the microbiology area. The responsibility of testing the efficacy of UV lamp lies with the Microbiologist/Analyst. Microbiology , microbial challenge , UV lamp

To lay down the Procedure for Isolation, Identification and Gram Staining of Microorganisms. This procedure applies to isolation, identification and gram staining of microorganisms during the course of regular microbiological analysis. The responsibility of isolation, identification and gram staining of microorganisms lies with the Microbiologist/Analyst. Microbiology , Isolation, Identification, Microorganisms

To lay down the Procedure for Entry and Exit of Microbiology Testing area. This procedure applies to entry and exit of Microbiology testing area for Microbiologist/Analyst, Lab boy and Maintenance personnel to carry out Microbiological tests, for cleaning of testing and change room and Maintenance works respectively Microbiology, Testing area , Entry and Exit, cleaning, Microbiological tests

 To lay down the procedure for sub culturing of microorganisms from pure cultures and preparation of standardized viable culture suspension. This procedure applies to preparation of, viable culture suspension, and subculturing and maintenance of the following cultures for the given organisms against the media used and incubation conditions in microbiology laboratory Microbiology , sub culturing , microorganisms, pure cultures , viable culture