Operation Of HPLC with LC Solution Software

To lay down a procedure for Operation of HPLC (Shimadzu with LC 10AT and SPD-M10 AVP). This SOP is applicable for HPLC (Shimadzu with LC 10AT and SPD-M10 AVP) in quality control department. Connect the instrument to 230 volts A/C power supply.
Switch on the instrument pumps A and B. Connect the required column for sample analysis and equilibrated for ten minutes with water/methanol or acetonitrile.
LC Solution, acetonitrile, maximum, minimum, acetonitrile, equilibrated
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